A Study on Image Quality in Polarization-Resolved Second Harmonic Generation Microscopy.

ABSTRACT: Second harmonic generation (SHG) microscopy represents a very powerful tool for tissue characterization. Polarization-resolved SHG (PSHG) microscopy extends the potential of SHG, by exploiting the dependence of SHG signals on the polarization state of the excitation beam. Among others, this dependence translates to the fact that SHG images collected under different polarization configurations exhibit distinct characteristics in terms of content and appearance. These characteristics hold deep implications over image quality, as perceived by human observers or by image analysis methods custom designed to automatically extract a quality factor from digital images. Our work addresses this subject, by investigating how basic image properties and the outputs of no-reference image quality assessm

SUBMITTER: Stanciu SG 

PROVIDER: S-EPMC5684207 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature