Nitric oxide mediated inhibition of antigen presentation from DCs to CD4⁺ T cells in cancer and measurement of STAT1 nitration.

ABSTRACT: Myeloid derived suppressor cells (MDSC) produce nitric oxide (NO) and inhibit dendritic cell (DC) immune responses in cancer. DCs present cancer cell antigens to CD4⁺ T cells through Jak-STAT signal transduction. In this study, NO donors (SNAP and DETA-NONOate) inhibited DC antigen presentation. As expected, MDSC isolated from peripheral blood mononuclear cells (PBMC) from cancer patients produced high NO levels. We hypothesized that NO producing MDSC in tumor-bearing hosts would inhibit DC antigen presentation. Antigen presentation from DCs to CD4⁺ T cells (T cell receptor transgenic OT-II) was measured via a [³H]-thymidine incorporation proliferation assay. MDSC from melanoma tumor models decreased the levels of proliferation more than pancreatic cancer d

SUBMITTER: Markowitz J 

PROVIDER: S-EPMC5684213 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature