A conformational switch in initiation factor 2 controls the fidelity of translation initiation in bacteria.

ABSTRACT: Initiation factor (IF) 2 controls the fidelity of translation initiation by selectively increasing the rate of 50S ribosomal subunit joining to 30S initiation complexes (ICs) that carry an N-formyl-methionyl-tRNA (fMet-tRNA^fMet). Previous studies suggest that rapid 50S subunit joining involves a GTP- and fMet-tRNA^fMet-dependent "activation" of IF2, but a lack of data on the structure and conformational dynamics of 30S IC-bound IF2 has precluded a mechanistic understanding of this process. Here, using an IF2-tRNA single-molecule fluorescence resonance energy transfer signal, we directly observe the conformational switch that is associated with IF2 activation within 30S ICs that lack IF3. Based on these results, we propose a model of IF2 activation that reveals how GTP,

SUBMITTER: Caban K 

PROVIDER: S-EPMC5684235 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature