Essential role of mitochondrial Stat3 in p38^MAPK mediated apoptosis under oxidative stress.

ABSTRACT: Stat3 is an oncogene, frequently associated with malignant transformation. A body of evidence implicates that phospho-Stat3^Y705 contributes to its nucleic translocation, while phospho-Stat3^S727 leads to the accumulation in mitochondria. Both are of importance for tumor cell proliferation. In comparison to well-characterized signaling pathways interplaying with Stat3^Y705, little is known about Stat3^S727. In this work, we studied the influence of Stat3 deficiency on the viability of cells exposed to H₂O₂ or hypoxia using siRNA and CRISPR/Cas9 genome-editing. We found dysregulation of mitochondrial activity, which was associated with excessive ROS formation and reduced mitochondrial membrane potential, and observed a synergisti

SUBMITTER: Cheng X 

PROVIDER: S-EPMC5684365 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature