Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues.

ABSTRACT:

Background

Lymph node metastasis status is a prognostic factor for further lymph node involvement and for patient survival in breast cancer patients. Frozen section analysis of lymph nodes is a reliable method for detection of macro-metastases. However, this method is far less effective in detecting micro-metastases, requesting improved diagnostic procedures.

Methods

We investigated expression and truncation of ezrin in (i) sentinel lymph node metastases, (ii) unaffected axillary lymph nodes, (iii) primary breast tumors, and (iv) healthy glandular breast tissues using 2D gel electrophoresis, SDS-PAGE, and mass spectrometry in addition to Western blotting.

Results

Full-length ezrin (E1; amino acids 1-586) is present in all four investigated tissues. Two truncated ezrin

SUBMITTER: Rower C 

PROVIDER: S-EPMC5684437 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature