Comparative proteomic analysis between nitrogen supplemented and starved conditions in Magnaporthe oryzae.

ABSTRACT:

Background

Fungi are constantly exposed to nitrogen limiting environments, and thus the efficient regulation of nitrogen metabolism is essential for their survival, growth, development and pathogenicity. To understand how the rice blast pathogen Magnaporthe oryzae copes with limited nitrogen availability, a global proteome analysis under nitrogen supplemented and nitrogen starved conditions was completed.

Methods

M. oryzae strain 70-15 was cultivated in liquid minimal media and transferred to media with nitrate or without a nitrogen source. Proteins were isolated and subjected to unfractionated gel-free based liquid chromatography-tandem mass spectrometry (LC-MS/MS). The subcellular localization and function of the identified proteins were predicted using bioin

SUBMITTER: Oh Y 

PROVIDER: S-EPMC5684745 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature