Effect of efflux pump inhibitors on the susceptibility of Mycobacterium tuberculosis to isoniazid.

ABSTRACT:

Background

Mycobacterium can develop drug resistance (DR) by mutation of its existing gene. However, the existence of DR without mutation shows the need to look for an alternative mechanism such as the role of efflux pumps. In this study, we examined the effect of efflux pump inhibitors on isoniazid (INH) susceptibility in clinical isolates of Mycobacterium tuberculosis (Mtb).

Materials and methods

Resazurin microtiter assay was used to examine the effect of efflux pump inhibitors on minimum inhibitory concentration (MIC) levels of INH in eighteen Mtb clinical isolates.

Results

The observed reduction in INH-MIC was 2-16-fold in INH-resistant isolates with katG and inhA gene mutations, 2-8-fold in INH-resistant isolates without mutation and 2-4-fold in INH-sensitive is

SUBMITTER: Jaiswal I 

PROVIDER: S-EPMC5684805 | biostudies-literature | 2017 Nov-Dec

REPOSITORIES: biostudies-literature