Ultrasensitive detection of acute myeloid leukemia minimal residual disease using single molecule molecular inversion probes.

ABSTRACT: The identification of minimal residual disease is the primary diagnostic finding which predicts relapse in patients treated for acute myeloid leukemia. Ultrasensitive detection of minimal residual disease would enable better patient risk stratification and could open opportunities for early therapeutic intervention. Herein we apply single molecule molecular inversion probe capture, a technology combining multiplexed targeted sequencing with error correction schemes based on molecular barcoding, in order to detect mutations identifying minimal residual disease with ultrasensitive and quantitative precision. We designed a single molecule molecular inversion probe capture panel spanning >50 kb and targeting 32 factors relevant to acute myeloid leukemia pathogenesis. We demonstrate linearity a

SUBMITTER: Waalkes A 

PROVIDER: S-EPMC5685235 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature