DrLumi: An open-source package to manage data, calibrate, and conduct quality control of multiplex bead-based immunoassays data analysis.

ABSTRACT: Multiplex bead-based immunoassays are used to measure concentrations of several analytes simultaneously. These assays include control standard curves (SC) to reduce between-plate variability and normalize quantitation of analytes of biological samples. Suboptimal calibration might result in large random error and decreased number of samples with analyte concentrations within the limits of quantification. Suboptimal calibration may be a consequence of poor fitness of the functions used for the SC, the treatment of the background noise and the method used to estimate the limits of quantification. Currently assessment of fitness of curves is largely dependent on operator and that may add additional error. Moreover, there is no software to automate data managing and quality control. In this ar

SUBMITTER: Sanz H 

PROVIDER: S-EPMC5685631 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature