Sparse Labeling PELDOR Spectroscopy on Multimeric Mechanosensitive Membrane Channels.

ABSTRACT: Pulse electron paramagnetic resonance (EPR) is being applied to ever more complex biological systems comprising multiple subunits. Membrane channel proteins are of great interest as pulse EPR reports on functionally significant but distinct conformational states in a native environment without the need for crystallization. Pulse EPR, in the form of pulsed electron-electron double resonance (PELDOR), using site-directed spin labeling, is most commonly employed to accurately determine distances (in the nanometer range) between different regions of the structure. However, PELDOR data analysis is more challenging in systems containing more than two spins (e.g., homomultimers) due to distorting multispin effects. Without suppression of these effects, much of the information contained in PELDOR

SUBMITTER: Ackermann K 

PROVIDER: S-EPMC5685675 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature