Abnormal CYP11A1 gene expression induces excessive autophagy, contributing to the pathogenesis of preeclampsia.

ABSTRACT:

Objective

In this study, we investigated the exact mechanism by which excessive CYP11A1 expression impairs the placentation process and whether this causes preeclampsia (PE) in an in vivo model.

Setting and design

In order to study CYP11A1 overexpression, BeWo cells were transfected with CYP11A1. Pregnenolone, progesterone, and testosterone levels were measured by enzyme linked immunosorbent assays, and levels of autophagy markers were quantified by western blotting and immunofluorescence. Trophoblastic cell invasion was assessed using transwell assays; BeWo cells were treated with testosterone and an androgen receptor (AR) inhibitor (flutamide) to elucidate the invasion mechanism. An adenovirus overexpression rat model was established to investigate CYP11A1 overexpre

SUBMITTER: Pan T 

PROVIDER: S-EPMC5685712 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature