Ensembles of Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement.

ABSTRACT: Most kinesin motors move in only one direction along microtubules. Members of the kinesin-5 subfamily were initially described as unidirectional plus-end-directed motors and shown to produce piconewton forces. However, some fungal kinesin-5 motors are bidirectional. The force production of a bidirectional kinesin-5 has not yet been measured. Therefore, it remains unknown whether the mechanism of the unconventional minus-end-directed motility differs fundamentally from that of plus-end-directed stepping. Using force spectroscopy, we have measured here the forces that ensembles of purified budding yeast kinesin-5 Cin8 produce in microtubule gliding assays in both plus- and minus-end direction. Correlation analysis of pause forces demonstrated that individual Cin8 molecules produce additive f

SUBMITTER: Fallesen T 

PROVIDER: S-EPMC5685778 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature