Combinatorial regulation of the balance between dynein microtubule end accumulation and initiation of directed motility.

ABSTRACT: Cytoplasmic dynein is involved in a multitude of essential cellular functions. Dynein's activity is controlled by the combinatorial action of several regulatory proteins. The molecular mechanism of this regulation is still poorly understood. Using purified proteins, we reconstitute the regulation of the human dynein complex by three prominent regulators on dynamic microtubules in the presence of end binding proteins (EBs). We find that dynein can be in biochemically and functionally distinct pools: either tracking dynamic microtubule plus-ends in an EB-dependent manner or moving processively towards minus ends in an adaptor protein-dependent manner. Whereas both dynein pools share the dynactin complex, they have opposite preferences for binding other regulators, either the adaptor protein

SUBMITTER: Jha R 

PROVIDER: S-EPMC5686545 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature