Effects of mRNA secondary structure on the expression of HEV ORF2 proteins in Escherichia coli.

ABSTRACT:

Background

Viral protein expression in Escherichia coli (E. coli) is a powerful tool for structural/functional studies as well as for vaccine and diagnostics development. However, numerous factors such as codon bias, mRNA secondary structure and nucleotides distribution, have been indentified to hamper this heterologous expression.

Results

In this study, we combined computational and biochemical methods to analyze the influence of these factors on the expression of different segments of hepatitis E virus (HEV) ORF 2 protein and hepatitis B virus surface antigen (HBsAg). Three out of five HEV antigens were expressed while all three HBsAg fragments were not. The computational analysis revealed a significant difference in nucleotide distribution between expressed and non-expres

SUBMITTER: Behloul N 

PROVIDER: S-EPMC5686824 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature