Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system.

ABSTRACT:

Background

Corynebacterium glutamicum (C. glutamicum) has traditionally been used as a microbial cell factory for the industrial production of many amino acids and other industrially important commodities. C. glutamicum has recently been established as a host for recombinant protein expression; however, some intrinsic disadvantages could be improved by genetic modification. Gene editing techniques, such as deletion, insertion, or replacement, are important tools for modifying chromosomes.

Results

In this research, we report a CRISPR/Cas9 system in C. glutamicum for rapid and efficient genome editing, including gene deletion and insertion. The system consists of two plasmids: one containing a target-specific guide RNA and a homologous sequence to a target gene, the other expr

SUBMITTER: Peng F 

PROVIDER: S-EPMC5686833 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature