Molecular evolution of HIV-1 integrase during the 20 years prior to the first approval of integrase inhibitors.

ABSTRACT:

Background

Detailed knowledge of the evolutionary potential of polymorphic sites in a viral protein is important for understanding the development of drug resistance in the presence of an inhibitor. We therefore set out to analyse the molecular evolution of the HIV-1 subtype B integrase at the inter-patient level in Germany during a 20-year period prior to the first introduction of integrase strand inhibitors (INSTIs).

Methods

We determined 337 HIV-1 integrase subtype B sequences (amino acids 1-278) from stored plasma samples of antiretroviral treatment-naïve individuals newly diagnosed with HIV-1 between 1986 and 2006. Shannon entropy was calculated to determine the variability at each amino acid position. Time trends in the frequency of amino acid variants were identified

SUBMITTER: Meixenberger K 

PROVIDER: S-EPMC5686839 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature