Micropipette force probe to quantify single-cell force generation: application to T-cell activation.

ABSTRACT: In response to engagement of surface molecules, cells generate active forces that regulate many cellular processes. Developing tools that permit gathering mechanical and morphological information on these forces is of the utmost importance. Here we describe a new technique, the micropipette force probe, that uses a micropipette as a flexible cantilever that can aspirate at its tip a bead that is coated with molecules of interest and is brought in contact with the cell. This technique simultaneously allows tracking the resulting changes in cell morphology and mechanics as well as measuring the forces generated by the cell. To illustrate the power of this technique, we applied it to the study of human primary T lymphocytes (T-cells). It allowed the fine monitoring of pushing and pulling forc

SUBMITTER: Sawicka A 

PROVIDER: S-EPMC5687025 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature