The balance between Gα_i-Cdc42/Rac and Gα₁₂/₁₃-RhoA pathways determines endothelial barrier regulation by sphingosine-1-phosphate.

ABSTRACT: The bioactive sphingosine-1-phosphatephosphate (S1P) is present in plasma, bound to carrier proteins, and involved in many physiological processes, including angiogenesis, inflammatory responses, and vascular stabilization. S1P can bind to several G-protein-coupled receptors (GPCRs) activating a number of different signaling networks. At present, the dynamics and relative importance of signaling events activated immediately downstream of GPCR activation are unclear. To examine these, we used a set of fluorescence resonance energy transfer-based biosensors for different RhoGTPases (Rac1, RhoA/B/C, and Cdc42) as well as for heterotrimeric G-proteins in a series of live-cell imaging experiments in primary human endothelial cells. These experiments were accompanied by biochemical GTPase activi

SUBMITTER: Reinhard NR 

PROVIDER: S-EPMC5687037 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature