A gel-free proteomic-based method for the characterization of Bordetella pertussis clinical isolates.

ABSTRACT: Bordetella pertussis (Bp) is the etiologic agent of pertussis or whooping cough, a highly contagious respiratory disease occurring primarily in infants and young children. Although vaccine preventable, pertussis cases have increased over the years leading researchers to re-evaluate vaccine control strategies. Since bacterial outer membrane proteins, comprising the surfaceome, often play roles in pathogenesis and antibody-mediated immunity, three recent Bp circulating isolates were examined using proteomics to identify any potential changes in surface protein expression. Fractions enriched for outer membrane proteins were digested with trypsin and the peptides analyzed by nano liquid chromatography-electrospray ionization-mass spectrometry (nLC-ESI-MS), followed by database analysis to eluc

SUBMITTER: Williamson YM 

PROVIDER: S-EPMC5687064 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature