Transcriptome dynamics revealed by a gene expression atlas of the early Arabidopsis embryo.

ABSTRACT: During early plant embryogenesis, precursors for all major tissues and stem cells are formed. While several components of the regulatory framework are known, how cell fates are instructed by genome-wide transcriptional activity remains unanswered-in part because of difficulties in capturing transcriptome changes at cellular resolution. Here, we have adapted a two-component transgenic labelling system to purify cell-type-specific nuclear RNA and generate a transcriptome atlas of early Arabidopsis embryo development, with a focus on root stem cell niche formation. We validated the dataset through gene expression analysis, and show that gene activity shifts in a spatio-temporal manner, probably signifying transcriptional reprogramming, to induce developmental processes reflecting cell states

SUBMITTER: Palovaara J 

PROVIDER: S-EPMC5687563 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature