Low cost and open source multi-fluorescence imaging system for teaching and research in biology and bioengineering.

ABSTRACT: The advent of easy-to-use open source microcontrollers, off-the-shelf electronics and customizable manufacturing technologies has facilitated the development of inexpensive scientific devices and laboratory equipment. In this study, we describe an imaging system that integrates low-cost and open-source hardware, software and genetic resources. The multi-fluorescence imaging system consists of readily available 470 nm LEDs, a Raspberry Pi camera and a set of filters made with low cost acrylics. This device allows imaging in scales ranging from single colonies to entire plates. We developed a set of genetic components (e.g. promoters, coding sequences, terminators) and vectors following the standard framework of Golden Gate, which allowed the fabrication of genetic constructs in a combinator

SUBMITTER: Nunez I 

PROVIDER: S-EPMC5687719 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature