Selection for avian leukosis virus integration sites determines the clonal progression of B-cell lymphomas.

ABSTRACT: Avian leukosis virus (ALV) is a simple retrovirus that causes a wide range of tumors in chickens, the most common of which are B-cell lymphomas. The viral genome integrates into the host genome and uses its strong promoter and enhancer sequences to alter the expression of nearby genes, frequently inducing tumors. In this study, we compare the preferences for ALV integration sites in cultured cells and in tumors, by analysis of over 87,000 unique integration sites. In tissue culture we observed integration was relatively random with slight preferences for genes, transcription start sites and CpG islands. We also observed a preference for integrations in or near expressed and spliced genes. The integration pattern in cultured cells changed over the course of selection for oncogenic character

SUBMITTER: Malhotra S 

PROVIDER: S-EPMC5687753 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature