Active diffusion and advection in Drosophila oocytes result from the interplay of actin and microtubules.

ABSTRACT: Transport in cells occurs via a delicate interplay of passive and active processes, including diffusion, directed transport and advection. Despite progress in super-resolution microscopy, discriminating and quantifying these processes is a challenge, requiring tracking of rapidly moving, sub-diffraction objects in a crowded, noisy environment. Here we use differential dynamic microscopy with different contrast mechanisms to provide a thorough characterization of the dynamics in the Drosophila oocyte. We study the movement of vesicles and the elusive motion of a cytoplasmic F-actin mesh, a known regulator of cytoplasmic flows. We find that cytoplasmic motility constitutes a combination of directed motion and random diffusion. While advection is mainly attributed to microtubules, we find tha

SUBMITTER: Drechsler M 

PROVIDER: S-EPMC5688094 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature