Transient increases in intracellular calcium and reactive oxygen species levels in TCam-2 cells exposed to microgravity.

ABSTRACT: The effects of microgravity on functions of the human body are well described, including alterations in the male and female reproductive systems. In the present study, TCam-2 cells, which are considered a good model of mitotically active male germ cells, were used to investigate intracellular signalling and cell metabolism during exposure to simulated microgravity, a condition that affects cell shape and cytoskeletal architecture. After a 24 hour exposure to simulated microgravity, TCam-2 cells showed 1) a decreased proliferation rate and a delay in cell cycle progression, 2) increased anaerobic metabolism accompanied by increased levels of intracellular Ca2+, reactive oxygen species and superoxide anion and modifications in mitochondrial morphology. Interestingly, all these events were tr

SUBMITTER: Morabito C 

PROVIDER: S-EPMC5688167 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature