Growth Characteristics of Methanomassiliicoccus luminyensis and Expression of Methyltransferase Encoding Genes.

ABSTRACT: DNA sequence analysis of the human gut revealed the presence a seventh order of methanogens referred to as Methanomassiliicoccales. Methanomassiliicoccus luminyensis is the only member of this order that grows in pure culture. Here, we show that the organism has a doubling time of 1.8 d with methanol + H₂ and a growth yield of 2.4 g dry weight/mol CH₄. M. luminyensis also uses methylamines + H₂ (monomethylamine, dimethylamine, and trimethylamine) with doubling times of 2.1-2.3 d. Similar cell yields were obtained with equimolar concentrations of methanol and methylamines with respect to their methyl group contents. The transcript levels of genes encoding proteins involved in substrate utilization indicated increased amounts of mRNA from the

SUBMITTER: Kroninger L 

PROVIDER: S-EPMC5688252 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature