Metabolic pathways and genes identified by RNA-seq analysis of barley near-isogenic lines differing by allelic state of the Black lemma and pericarp (Blp) gene.

ABSTRACT:

Background

Some plant species have 'melanin-like' black seed pigmentation. However, the chemical and genetic nature of this 'melanin-like' black pigment have not yet been fully explored due to its complex structure and ability to withstand almost all solvents. Nevertheless, identification of genetic networks participating in trait formation is key to understanding metabolic processes involved in the expression of 'melanin-like' black seed pigmentation. The aim of the current study was to identify differentially expressed genes (DEGs) in barley near-isogenic lines (NILs) differing by allelic state of the Blp (black lemma and pericarp) locus.

Results

RNA-seq analysis of six libraries (three replicates for each line) was performed. A total of 957 genome fragments had statistica

SUBMITTER: Glagoleva AY 

PROVIDER: S-EPMC5688459 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature