Fine organization of genomic regions tagged to the 5S rDNA locus of the bread wheat 5B chromosome.

ABSTRACT:

Background

The multigene family encoding the 5S rRNA, one of the most important structurally-functional part of the large ribosomal subunit, is an obligate component of all eukaryotic genomes. 5S rDNA has long been a favored target for cytological and phylogenetic studies due to the inherent peculiarities of its structural organization, such as the tandem arrays of repetitive units and their high interspecific divergence. The complex polyploid nature of the genome of bread wheat, Triticum aestivum, and the technically difficult task of sequencing clusters of tandem repeats mean that the detailed organization of extended genomic regions containing 5S rRNA genes remains unclear. This is despite the recent progress made in wheat genomic sequencing. Using pyrosequencing of BAC clones,

SUBMITTER: Sergeeva EM 

PROVIDER: S-EPMC5688495 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature