Development of F1 hybrid population and the high-density linkage map for European aspen (Populus tremula L.) using RADseq technology.

ABSTRACT:

Background

Restriction-site associated DNA sequencing (RADseq) technology was recently employed to identify a large number of single nucleotide polymorphisms (SNP) for linkage mapping of a North American and Eastern Asian Populus species. However, there is also the need for high-density genetic linkage maps for the European aspen (P. tremula) as a tool for further mapping of quantitative trait loci (QTLs) and marker-assisted selection of the Populus species native to Europe.

Results

We established a hybrid F1 population from the cross of two aspen parental genotypes diverged in their phenological and morphological traits. We performed RADseq of 122 F1 progenies and two parents yielding 15,732 high-quality SNPs that were successfully identified using the reference genome of P

SUBMITTER: Zhigunov AV 

PROVIDER: S-EPMC5688504 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature