MicroRNA profiling of dogs with transitional cell carcinoma of the bladder using blood and urine samples.

ABSTRACT:

Background

Early signs of canine transitional cell carcinoma (TCC) are frequently assumed to be caused by other lower urinary tract diseases (LUTD) such as urinary tract infections, resulting in late diagnosis of TCC which could be fatal. The development of a non-invasive clinical test for TCC could dramatically reduce mortality. To determine whether microRNAs (miRNAs) can be used as non-invasive diagnostic biomarkers, we assessed miRNA expression in blood and/or urine from dogs with clinically normal bladders (n = 28), LUTD (n = 25), and TCC (n = 17). Expression levels of 5 miRNA associated with TCC pathophysiology (miR-34a, let-7c, miR-16, miR-103b, and miR-106b) were assessed by quantitative real-time PCR.

Results

Statistical analyses using ranked ANOVA identified signifi

SUBMITTER: Kent MS 

PROVIDER: S-EPMC5688639 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature