Usability of human Infinium MethylationEPIC BeadChip for mouse DNA methylation studies.

ABSTRACT:

Background

The advent of array-based genome-wide DNA methylation methods has enabled quantitative measurement of single CpG methylation status at relatively low cost and sample input. Whereas the use of Infinium Human Methylation BeadChips has shown great utility in clinical studies, no equivalent tool is available for rodent animal samples. We examined the feasibility of using the new Infinium MethylationEPIC BeadChip for studying DNA methylation in mouse.

Results

In silico, we identified 19,420 EPIC probes (referred as mEPIC probes), which align with a unique best alignment score to the bisulfite converted reference mouse genome mm10. Further annotation revealed that 85% of mEPIC probes overlapped with mm10.refSeq genes at different genomic features including promoters (TS

SUBMITTER: Needhamsen M 

PROVIDER: S-EPMC5688710 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature