Patient-derived xenograft in zebrafish embryos: a new platform for translational research in gastric cancer.

ABSTRACT:

Background

Gastric cancer (GC) is among the most commonly cancer occurred in Asian, especially in China. With its high heterogeneity and few of validated drug targets, GC remains to be one of the most under explored areas of precision medicine. In this study, we aimed to establish an in vivo patient-derived xenograft (PDX) model based on zebrafish (Danio rerio) embryos, allowing for a rapid analysis of the angiogenic and invasive potentials, as well as a fast drug sensitivity testing.

Methods

Two human gastric cancer cell lines (AGS and SGC-7901) were xenografted into zebrafish embryos, their sensitivity to 5-FU were tested both in vitro and in vivo. Fourteen human primary cells from gastric cancer tissue were xenografted into zebrafish embryos, their proliferating, angiogen

SUBMITTER: Wu JQ 

PROVIDER: S-EPMC5688753 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature