Light induced expression of β-glucosidase in Escherichia coli with autolysis of cell.

ABSTRACT:

Background

β-Glucosidase has attracted substantial attention in the scientific community because of its pivotal role in cellulose degradation, glycoside transformation and many other industrial processes. However, the tedious and costly expression and purification procedures have severely thwarted the industrial applications of β-glucosidase. Thus development of new strategies to express β-glucosidases with cost-effective and simple procedure to meet the increasing demands on enzymes for biocatalysis is of paramount importance.

Results

Light activated cassette YF1/FixJ and the SRRz lysis system were successfully constructed to produce Bgl1A(A24S/F297Y), a mutant β-glucosidase tolerant to both glucose and ethanol. By optimizing the parameters for light induction, Bgl1A(A24S/F

SUBMITTER: Chang F 

PROVIDER: S-EPMC5688802 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature