Calcium-containing scaffolds induce bone regeneration by regulating mesenchymal stem cell differentiation and migration.

ABSTRACT:

Background

Osteoinduction and subsequent bone formation rely on efficient mesenchymal stem cell (MSC) recruitment. It is also known that migration is induced by gradients of growth factors and cytokines. Degradation of Ca²⁺-containing biomaterials mimics the bone remodeling compartment producing a localized calcium-rich osteoinductive microenvironment. The aim of our study was to determine the effect of calcium sulfate (CaSO₄) on MSC migration. In addition, to evaluate the influence of CaSO₄ on MSC differentiation and the potential molecular mechanisms involved.

Methods

A circular calvarial bone defect (5 mm diameter) was created in the parietal bone of 35 Balb-C mice. We prepared and implanted a cell-free agarose/gelatin scaffold alone or in

SUBMITTER: Aquino-Martinez R 

PROVIDER: S-EPMC5689169 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature