Hybrid de novo genome assembly and centromere characterization of the gray mouse lemur (Microcebus murinus).

ABSTRACT:

Background

The de novo assembly of repeat-rich mammalian genomes using only high-throughput short read sequencing data typically results in highly fragmented genome assemblies that limit downstream applications. Here, we present an iterative approach to hybrid de novo genome assembly that incorporates datasets stemming from multiple genomic technologies and methods. We used this approach to improve the gray mouse lemur (Microcebus murinus) genome from early draft status to a near chromosome-scale assembly.

Methods

We used a combination of advanced genomic technologies to iteratively resolve conflicts and super-scaffold the M. murinus genome.

Results

We improved the M. murinus genome assembly to a scaffold N50 of 93.32 Mb. Whole genome alignments between our primary su

SUBMITTER: Larsen PA 

PROVIDER: S-EPMC5689209 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature