Large-Scale Ex Vivo Generation of Human Red Blood Cells from Cord Blood CD34⁺ Cells.

ABSTRACT: The ex vivo generation of human red blood cells on a large scale from hematopoietic stem and progenitor cells has been considered as a potential method to overcome blood supply shortages. Here, we report that functional human erythrocytes can be efficiently produced from cord blood (CB) CD34⁺ cells using a bottle turning device culture system. Safety and efficiency studies were performed in murine and nonhuman primate (NHP) models. With the selected optimized culture conditions, one human CB CD34⁺ cell could be induced ex vivo to produce up to 200 million erythrocytes with a purity of 90.1% ± 6.2% and 50% ± 5.7% (mean ± SD) for CD235a⁺ cells and enucleated cells, respectively. The yield of erythrocytes from one CB unit (5 million CD34⁺ cells) cou

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5689780 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature