Mitosis-Mediated Intravasation in a Tissue-Engineered Tumor-Microvessel Platform.

ABSTRACT: Intravasation involves the migration of tumor cells across the local endothelium and escape into vessel flow. Although tumor cell invasiveness has been correlated to increased intravasation, the details of transendothelial migration and detachment into circulation are still unclear. Here, we analyzed the intravasation of invasive human breast cancer cells within a tissue-engineered microvessel model of the tumor microenvironment. Using live-cell fluorescence microscopy, we captured 2,330 hours of tumor cell interactions with functional microvessels and provide evidence for a mitosis-mediated mechanism where tumor cells located along the vessel periphery are able to disrupt the vessel endothelium through cell division and detach into circulation. This model provides a framework for understa

SUBMITTER: Wong AD 

PROVIDER: S-EPMC5690825 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature