A single nucleotide polymorphism of cyclin-dependent kinase inhibitor 1B (p27^Kip1) associated with human vein graft failure affects growth of human venous adventitial cells but not smooth muscle cells.

ABSTRACT:

Background

Cyclin-dependent kinase inhibitor 1B (p27^Kip1) is a cell-cycle inhibitor whose -838C>A single nucleotide polymorphism (rs36228499; hereafter called p27 SNP) has been associated with the clinical failure of peripheral vein grafts, but the functional effects of this SNP have not been demonstrated.

Methods

Human saphenous vein adventitial cells and intimal/medial smooth muscle cells (SMCs) were derived from explants obtained at the time of lower extremity bypass operations. We determined the following in adventitial cells and SMCs as a function of the p27 SNP genotype: (1) p27 promoter activity, (2) p27 messenger (m)RNA and protein levels, and (3) growth and collagen gel contraction. Deoxyribonuclease I footprinting was also performed in adventitial cells

SUBMITTER: Kenagy RD 

PROVIDER: S-EPMC5690896 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature