Regulation of gene expression and RNA editing in Drosophila adapting to divergent microclimates.

ABSTRACT: Determining the mechanisms by which a species adapts to its environment is a key endeavor in the study of evolution. In particular, relatively little is known about how transcriptional processes are fine-tuned to adjust to different environmental conditions. Here we study Drosophila melanogaster from 'Evolution Canyon' in Israel, which consists of two opposing slopes with divergent microclimates. We identify several hundred differentially expressed genes and dozens of differentially edited sites between flies from each slope, correlate these changes with genetic differences, and use CRISPR mutagenesis to validate that an intronic SNP in prominin regulates its editing levels. We also demonstrate that while temperature affects editing levels at more sites than genetic differences, geneticall

SUBMITTER: Yablonovitch AL 

PROVIDER: S-EPMC5691062 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature