Single molecule multiplexed nanopore protein screening in human serum using aptamer modified DNA carriers.

ABSTRACT: The capability to screen a range of proteins at the single-molecule level with enhanced selectivity in biological fluids has been in part a driving force in developing future diagnostic and therapeutic strategies. The combination of nanopore sensing and nucleic acid aptamer recognition comes close to this ideal due to the ease of multiplexing, without the need for expensive labelling methods or extensive sample pre-treatment. Here, we demonstrate a fully flexible, scalable and low-cost detection platform to sense multiple protein targets simultaneously by grafting specific sequences along the backbone of a double-stranded DNA carrier. Protein bound to the aptamer produces unique ionic current signatures which facilitates accurate target recognition. This powerful approach allows us to diff

SUBMITTER: Sze JYY 

PROVIDER: S-EPMC5691071 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature