QTL mapping and molecular characterization of the classical D locus controlling seed and flower color in Linum usitatissimum (flax).

ABSTRACT: The flowers of flax (linseed) are blue-hued, ephemeral and self-pollinating, and the seeds are typically brown. A century-old interest in natural yellow seed variants and a historical model point to recessive alleles in B1, D and G loci being responsible, but the functional aspects had remained unknown. Here, we characterized the "D" locus by quantitative trait loci (QTL) mapping and identified a FLAVONOID 3'5' HYDROXYLASE (F3'5'H) gene therein. It does not belong to the F3'5'H clade, but resembles biochemically characterized F3'Hs (flavonoid 3' hydroxylase) but without F3'H activity. The genome lacks other F3'H or F3'H-like genes. The apparent neo-functionalization from F3'H is associated with a Thr₄₉₈ → Ser₄₉₈ substitution in a substrate recognition site (SRS). The

SUBMITTER: Sudarshan GP 

PROVIDER: S-EPMC5691222 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature