Engaging Ly-6A/Sca-1 triggers lipid raft-dependent and -independent responses in CD4⁺ T-cell lines.

ABSTRACT:

Introduction

The lymphocyte antigen 6 (Ly-6) supergene family encodes proteins of 12-14 kda in molecular mass that are either secreted or anchored to the plasma membrane through a glycosyl-phosphatidylinisotol (GPI) lipid anchor at the carboxy-terminus. The lipidated GPI-anchor allows localization of Ly-6 proteins to the 10-100 nm cholesterol-rich nano-domains on the membrane, also known as lipid rafts. Ly-6A/Sca-1, a member of Ly-6 gene family is known to transduce signals despite the absence of transmembrane and cytoplasmic domains. It is hypothesized that the localization of Ly-6A/Sca-1 with in lipid rafts allows this protein to transduce signals to the cell interior.

Methods and results

In this study, we found that cross-linking mouse Ly-6A/Sca-1 protein with a monoclona

SUBMITTER: Lang MA 

PROVIDER: S-EPMC5691314 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature