Concomitant Loss of the Glyoxalase System and Glycolysis Makes the Uncultured Pathogen "Candidatus Liberibacter asiaticus" an Energy Scavenger.

ABSTRACT: Methylglyoxal (MG) is a cytotoxic, nonenzymatic by-product of glycolysis that readily glycates proteins and DNA, resulting in carbonyl stress. Glyoxalase I and II (GloA and GloB) sequentially convert MG into d-lactic acid using glutathione (GSH) as a cofactor. The glyoxalase system is essential for the mitigation of MG-induced carbonyl stress, preventing subsequent cell death, and recycling GSH for maintenance of cellular redox poise. All pathogenic liberibacters identified to date are uncultured, including "Candidatus Liberibacter asiaticus," a psyllid endosymbiont and causal agent of the severely damaging citrus disease "huanglongbing." In silico analysis revealed the absence of gloA in "Ca Liberibacter asiaticus" and all other pathogenic liberibacters. Both <

SUBMITTER: Jain M 

PROVIDER: S-EPMC5691416 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature