Generation of a platform strain for ionic liquid tolerance using adaptive laboratory evolution.

ABSTRACT:

Background

There is a need to replace petroleum-derived with sustainable feedstocks for chemical production. Certain biomass feedstocks can meet this need as abundant, diverse, and renewable resources. Specific ionic liquids (ILs) can play a role in this process as promising candidates for chemical pretreatment and deconstruction of plant-based biomass feedstocks as they efficiently release carbohydrates which can be fermented. However, the most efficient pretreatment ILs are highly toxic to biological systems, such as microbial fermentations, and hinder subsequent bioprocessing of fermentative sugars obtained from IL-treated biomass.

Methods

To generate strains capable of tolerating residual ILs present in treated feedstocks, a tolerance adaptive laboratory evolution (TALE)

SUBMITTER: Mohamed ET 

PROVIDER: S-EPMC5691611 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature