Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group.

ABSTRACT: The Puccinia graminis f. sp. tritici (Pgt) Ug99 race group is virulent to most stem rust resistance genes currently deployed in wheat and poses a threat to global wheat production. The durum wheat (Triticum turgidum ssp. durum) gene Sr13 confers resistance to Ug99 and other virulent races, and is more effective at high temperatures. Using map-based cloning, we delimited a candidate region including two linked genes encoding coiled-coil nucleotide-binding leucine-rich repeat proteins designated CNL3 and CNL13. Three independent truncation mutations identified in each of these genes demonstrated that only CNL13 was required for Ug99 resistance. Transformation of an 8-kb genomic sequence including CNL13 into the susceptible wheat variety

SUBMITTER: Zhang W 

PROVIDER: S-EPMC5692537 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature