Induction of H3K9me3 and DNA methylation by tethered heterochromatin factors in Neurospora crassa.

ABSTRACT: Functionally different chromatin domains display distinct chemical marks. Constitutive heterochromatin is commonly associated with trimethylation of lysine 9 on histone H3 (H3K9me3), hypoacetylated histones, and DNA methylation, but the contributions of and interplay among these features are not fully understood. To dissect the establishment of heterochromatin, we investigated the relationships among these features using an in vivo tethering system in Neurospora crassa Artificial recruitment of the H3K9 methyltransferase DIM-5 (defective in methylation-5) induced H3K9me3 and DNA methylation at a normally active, euchromatic locus but did not bypass the requirement of DIM-7, previously implicated in the localization of DIM-5, indicating additional DIM-7 functionality. Tethered hetero

SUBMITTER: Gessaman JD 

PROVIDER: S-EPMC5692599 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature