Tackling Bet v 1 and associated food allergies with a single hybrid protein.

ABSTRACT:

Background

Allergy vaccines should be easily applicable, safe, and efficacious. For Bet v 1-mediated birch pollen and associated food allergies, a single wild-type allergen does not provide a complete solution.

Objective

We aimed to combine immunologically relevant epitopes of Bet v 1 and the 2 clinically most important related food allergens from apple and hazelnut to a single hybrid protein, termed MBC4.

Methods

After identification of T cell epitope-containing parts on each of the 3 parental allergens, the hybrid molecule was designed to cover relevant epitopes and evaluated in silico. Thereby a mutation was introduced into the hybrid sequence, which should alter the secondary structure without compromising the immunogenic properties of the molecule.

Results

SUBMITTER: Hofer H 

PROVIDER: S-EPMC5693327 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature