Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells.

ABSTRACT:

Background

Current approaches for quantification of major energy-storage forms in microalgae, including starch, protein and lipids, generally require cell cultivation to collect biomass followed by tedious and time-consuming analytical procedures. Thus, label-free, non-destructive and simultaneous quantification of such macromolecules at single-cell resolution is highly desirable in microalgal feedstock development and bioprocess control.

Results

Here, we established a method based on single-cell Raman spectra (SCRS) that simultaneously quantifies the contents of starch, protein, triacylglycerol (TAG) and lipid unsaturation degree in individual Chlamydomonas reinhardtii cells. Measurement accuracy for the contents based on full SCRS spectrum each reached 96.86-99.24%,

SUBMITTER: He Y 

PROVIDER: S-EPMC5693592 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature