Influence of Molecular size on the clearance of antibody fragments.

ABSTRACT:

Purpose

To establish a continuous relationship between the size of various antibody fragments and their systemic clearance (CL) in mice.

Methods

Two different orthogonal approaches have been used to establish the relationship. First approach uses CL values estimated by non-compartmental analysis (NCA) to establish a correlation with protein size. The second approach simultaneously characterizes the PK data for all the proteins using a 2-compartment model to establish a relationship between protein size and pharmacokinetic (PK) parameters.

Results

Simple mathematical functions (e.g. sigmoidal, power law) were able to characterize the CL vs. protein size relationship generated using the investigated proteins. The relationship established in mouse was used to predict rat

SUBMITTER: Li Z 

PROVIDER: S-EPMC5693627 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature