Optimization of ¹H decoupling eliminates sideband artifacts in 3D TROSY-based triple resonance experiments.

ABSTRACT: TROSY-based triple resonance experiments are essential for protein backbone assignment of large biomolecular systems by solution NMR spectroscopy. In a survey of the current Bruker pulse sequence library for TROSY-based experiments we found that several sequences were plagued by artifacts that affect spectral quality and hamper data analysis. Specifically, these experiments produce sidebands in the ¹³C(t ₁) dimension with inverted phase corresponding to ¹H_N resonance frequencies, with approximately 5% intensity of the parent ¹³C crosspeaks. These artifacts originate from the modulation of the ¹H_N frequency onto the resonance frequency of ¹³Cα and/or ¹³Cβ and are due to 180° pulses imperfectio

SUBMITTER: Xia Y 

PROVIDER: S-EPMC5693675 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature