Topological Structure Determination of RNA Using Small-Angle X-Ray Scattering.

ABSTRACT: Knowledge of RNA three-dimensional topological structures provides important insight into the relationship between RNA structural components and function. It is often likely that near-complete sets of biochemical and biophysical data containing structural restraints are not available, but one still wants to obtain knowledge about approximate topological folding of RNA. In this regard, general methods for determining such topological structures with minimum readily available restraints are lacking. Naked RNAs are difficult to crystallize and NMR spectroscopy is generally limited to small RNA fragments. By nature, sequence determines structure and all interactions that drive folding are self-contained within sequence. Nevertheless, there is little apparent correlation between primary sequenc

SUBMITTER: Bhandari YR 

PROVIDER: S-EPMC5693684 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature